详细信息

RNAzol® RT
RNAzol RT—新的small RNA提取试剂
◆ 可同时分离"total RNA"与"small RNA"，RNA提取的新突破
◆ 全新萃取方式，革命性技术突破，省去繁复的分层步骤
◆ 只需加 "水" 即可沈淀 RNA，*不须有机溶剂
◆ 可同时分离 "total RNA" 与 "small RNA"
◆ 免分层、免用有机溶剂，操作方便又安全
◆ 全程可于室温完成
◆ 低 DNA 污染，不须 DNase 处理
◆ 可单独纯化 microRNA

订购信息

目录号	产品说明	规格/单位	价格
RN 190	RNAzol® RT	50 ml	900
RN 190	RNAzol® RT	100 ml	1640
RN 190	RNAzol® RT	200 ml	2960
RN 190	RNAzol® RT	500 ml	7300


RNAzol®RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent （1） provides higher yield and quality of isolated RNA than previous reagents based on the single-step method （2,3）. RNAzol®RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.
RNAzol®RT separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. A biological sample is homogenized or lysed in RNAzol®RT. DNA, proteins, polysaccharides and other molecules are precipitated from the homogenate/lysate by the addition of water and removed by centrifugation. The pure RNA is isolated from the resulting supernatant by alcohol precipitation, followed by washing and solubilization.
- The isolation procedure can be completed in less than one hour. The isolated RNA is ready for use in RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
- RNAzol®RT isolates mRNA and micro RNA in separate fractions. Alternatively, total RNA containing all classes of RNA in a single fraction can be isolated. In addition, RNAzol®RT allows for the sequential isolation of RNA and DNA.
- The RNAzol®RT procedure is performed at room temperature, including centrifugation.